HeLa or MCF-7 cells were grown in Dubelcco's modified Eagle's medium (DMEM) supplemented with 10% heat inactivated fetal calf serum. For transient expression, one day before transfection with the appropriate DNA vector, exponentially growing cells were seeded at a density of 1.56106 cells/78 cm2. According to the LipofectamineTM reagent …
Get QuoteFurthermore, the scaffold did not show a cytotoxic effect on A-375, MCF-7, and MDA--468, and its surface was hydrophobic as the water contact angle with the surface was 136.5°. The prepared prototype with natural ingredients and high efficacy could be considered for further consideration in vivo study or complementary medicine.
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Get QuoteThe compound 5a exhibited higher activity with an IC 50 value of 10.26 ± 0.71 µM, compared to 5d with an IC 50 value of 11.56 ± 1.98 µM for the HT-1080 and MCF-7 cancer cells line, respectively. Moreover, Annexin-V apoptosis was assessed by flow cytometry for hybrid compounds 5a and 5d against HT-1080 and MCF-7 competitor cell lines, as ...
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Get QuoteSASP promotes neuroendocrine transdifferentiation in MCF‐7 breast cancer cells. MCF‐7 cells were treated every 3 days with control (CTL) or SASP conditioned medium for 6 days. (a) Bright‐field micrographs of MCF‐7 cell morphology (representative of more than 5 independent experiments).
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